Respiration Activity in Flexibacter Chinensis under Starvation Stress
نویسنده
چکیده
Electron transport system (ETS) activity was found to be related to the initial inoculum size, culturability and viability of E. coli. In this experiment, ETS activity of Flexibacter chinensis was measured by INT-method under a variety of conditions such as starvation media with different amendments and different temperatures. The relation between ETS activity, cell culturability, and viability was investigated. We found that the addition of glucose increased ETS activity without affecting cell culturability. Urea addition led to an increase in survival, but did not increase ETS activity.
منابع مشابه
The Effect of Starvation Stress on the Protein Profiles in Flexibacter chinensis
Background: Analysis of many proteins produced during the transition into the stationary phase and under stress conditions (including starvation stress) demonstrated that a number of novel proteins were induced in common to each stress and could be the reason for cross-protection in bacterial cells. It is necessary to investigate the synthesis of these proteins during different stress condition...
متن کاملThe effect of starvation stress on the protein profiles in Flexibacter chinensis.
BACKGROUND Analysis of many proteins produced during the transition into the stationary phase and under stress conditions (including starvation stress) demonstrated that a number of novel proteins were induced in common to each stress and could be the reason for cross-protection in bacterial cells. It is necessary to investigate the synthesis of these proteins during different stress conditions...
متن کاملThe Effect of Some Environmental Factors on the Survival Characteristics of Flexibacter chinensis
To survive in natural waters, bacteria must respond to variety of environmental factors. Flexibacter survival in the aquatic environment is influenced by number of parameters, such as temperature, heavy metal concentration and oxidation. Here we investigate the influence of these factors on the survival of Flexibacter chinensis in starvation medium. Our data shows that different concentrations ...
متن کاملThe Mutation of the rpoS Gene, the Central Regulator of Stationary Phase, Affects the Cell Division in Flexibacter chinensis
A one kb portion of the rpoS gene from Flexibacter chinensis was isolated by PCR, sequenced and compared to the rpoS gene of a variety of other organisms. The gene was found to be 98% similar to previously sequenced genes. Mutation of the rpoS gene with tri-parental mating produced strain JR101 and the growth rate of the mutant was compared with that of the wild-type. The mutant grew slower, an...
متن کامل-
A Sigma factor gene which is important in the global regulation of heat shock responses in prokaryotes, rpoH, was isolated from Flexibacter chinesis by PCR, sequenced and compared to the rpoH genes of a variety of other organisms. The rpoH gene was 98% similar to other previously characterized genes. A mutant of rpoH was produced (JR102) which had a reduced growth rate at both low and high temp...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره شماره
صفحات -
تاریخ انتشار 2008